General Cell Based Assays
Meso Scale Discovery U-PLEX Human IP-10 ASY
The Meso Scale Discovery U-PLEX Human IP-10 Assay is an electrochemiluminescent immunoassay for the quantitative measurement of human interferon gamma-induced protein 10 (IP-10) in serum plasma and cell culture supernatants It utilizes MSD s proprietary SECTOR Imager 6000 for high sensitivity and a wide dynamic range The assay has high specificity with no cross-reactivity and 1% interference It requires 50 L or less sample volume and has an ultra-low lower limit of detection of 45 fg/mL The assay takes 3 5 hours for completion This powerful and sensitive assay allows reliable quantification of IP-10 across a wide range of concentrations
Meso Scale Discovery U-PLEX Mouse TNF-RI ASY
The Meso Scale Discovery U-PLEX Mouse TNF RI Assay Kit is a multiplex immunoassay that measures mouse tumor necrosis factor receptor I (TNF RI) in serum plasma and cell culture supernatants It uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with an ECL tag calibrators and buffer for sample dilution It can test up to 80 samples in duplicate and has a testing time of 3 5 hours The assay has high specificity and shows minimal cross-reactivity
Apexbio Technology LLC Live-Dead Cell Staining Kit, 500T. Cas: MFCD: NA.
Our product Live-Dead Cell Staining Kit is used for simultaneous fluorescence staining of viable and dead cells. This kit contains two dyes - Calcein-AM and Propidium Iodide (PI), which stain viable and dead cells, respectively.
Eurofins Discoverx PathHunter eXpress GPR27 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0334E2ACP1L)
The PathHunter GPR27 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR27 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR27 and EA-tagged β-Arrestin-2. Activation of GPR27 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter NPY2R Bioassay Kit, 10-Plate. (95-0077Y2-00056)
The cAMP Hunter NPY2R Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. peptide YY) based activation of NPY2R GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type NPY2R, a naturally Gi coupled GPCR. Upon activation of NPY2R, the (decrease/increase) in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter PD-1 Signaling Bioassay Kit, 2pl. (93-1104Y19-00117)
The PathHunter PD-1 Signaling Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This cell line enables assessment of ligand (e.g. PD-L1, PD-L2, activating antibody) based activation of PD-1 receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged PD-1 target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter Bioassay Kit ED Detection Kit, 10 (200 dp, 96-well). (93-1043E)
The PathHunter Bioassay ED Detection Kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter Bioassay cells overexpressing ED (ProLabel/PK) but not containing any EA. This kit contains lysis buffer, exogenous EA, and EFC substrate to allow for detection of levels of ED in the assayed cells. This kit contains all components required to assay 2 plates (96-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-1043e.
Eurofins Discoverx PathHunter IL17RA/IL17RC Dimerization Bioassay Kit, 2pl. (93-0999Y3-00053)
The PathHunter IL17RA/IL17RC Dimerization Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-17) induced dimerization of IL17RA/IL17RC receptors. This product uses EFC technology. The cell line included overexpresses IL17RC target protein fused to PK alongside IL17RA target protein fused to EA. Upon ligand-induced dimerization of IL17RA/IL17RC, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter SIRPa Signaling Bioassay Kit, 2pl. (93-1135Y19-00129)
The PathHunter SIRPa Signaling Bioassay consists of human cells engineered to measure drug potency and detect neutralizing antibodies. Engagement of the receptor by its ligand, or agonist antibodies results in phosphorylation of the receptor’s cytosolic tail. The SH2-domain fused to EA binds the phosphorylated receptor, forcing complementation of ED and EA, resulting in formation of an active β-gal enzyme, which hydrolyzes the substrate to generate a chemiluminescent signal. The bioassay kit contains all reagents needed for the assay including pre-qualified cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter Bioassay Kit ED Detection Kit, 50 (1,000 dp, 96-well). (93-1043)
The PathHunter Bioassay ED Detection Kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter Bioassay cells overexpressing ED (ProLabel/PK) but not containing any EA. This kit contains lysis buffer, exogenous EA, and EFC substrate to allow for detection of levels of ED in the assayed cells. This kit contains all components required to assay 10 plates (96-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-1043.
Eurofins Discoverx PathHunter eXpress PTHR1 Activated GPCR Internalization Assay, 2pl Kit. (93-0770E3CP0M)
The PathHunter PTHR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of PTHR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged PTHR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting PTHR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OXGR1 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0693E2CP1M)
The PathHunter OXGR1 (GPR80, GPR99) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of OXGR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OXGR1 and EA-tagged β-Arrestin-2. Activation of OXGR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter Exenatide Bioassay Kit, 10-Plate. (95-0062Y2-00102)
The cAMP Hunter Exenatide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Exenatide based activation of GLP1R GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP1R, a naturally Gs coupled GPCR. Upon activation of GLP1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress FPR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0314E2CP0M)
The PathHunter FPR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. formylpeptides) based activation of FPR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged FPR1 and EA-tagged β-Arrestin-2. Activation of FPR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter Exenatide Bioassay Kit, 2-Plate. (95-0062Y2-00101)
The cAMP Hunter Exenatide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Exenatide based activation of GLP1R GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP1R, a naturally Gs coupled GPCR. Upon activation of GLP1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.