General Cell Based Assays
Eurofins Discoverx PathHunter eXpress UTR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0283E2CP2M)
The PathHunter UTR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. urotensin) based activation of UTR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged UTR2 and EA-tagged β-Arrestin-2. Activation of UTR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress F2R (PAR1) CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0278E2CP0L)
The PathHunter F2R (PAR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. proteases, TFLLRN) based activation of F2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged F2R and EA-tagged β-Arrestin-2. Activation of F2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress NMU1R U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0232E3CP0L)
The PathHunter nMU1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. neuromedin U) based activation of nMU1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged nMU1R and EA-tagged β-Arrestin-2. Activation of nMU1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress BAI3 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0547E2ACP1M)
The PathHunter BAI3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of BAI3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged BAI3 and EA-tagged β-Arrestin-2. Activation of BAI3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress SSTR2 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0095E2CP2L)
The cAMP Hunter SSTR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin 28) based activation of SSTR2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type SSTR2, a naturally Gi/Go coupled GPCR. Upon activation of SSTR2, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress DRD5 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0094E2CP2M)
The cAMP Hunter DRD5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD5 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type DRD5, a naturally Gs coupled GPCR. Upon activation of DRD5, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR141 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0385E2ACP1M)
The PathHunter GPR141 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR141 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR141 and EA-tagged β-Arrestin-2. Activation of GPR141 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress DRD2S (Short Isoform) CHO-K1 β-Arrestin GPCR Assay, 2pl Kit. (93-0351E2CP0M)
The PathHunter DRD2S eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD2S GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged DRD2S and EA-tagged β-Arrestin-2. Activation of DRD2S stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CALCR U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0566E3CP5M)
The PathHunter CALCR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. calcitonin) based activation of CALCR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CALCR and EA-tagged β-Arrestin-2. Activation of CALCR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 7 Reagent, 500. (93-0563R7B)
AssayComplete Cell Plating 7 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Abcam Glycogen Colorimetric Assay Kit
Glycogen Colorimetric Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Molecular Devices CatchPoint cGMP Assay Kit, 384-Well, Clear Bottom
The CatchPoint cGMP Fluorescent Assay Kit Explorer configuration is a high-sensitivity, cell-compatible immunoassay for quantitative measurement of cyclic GMP in high-throughput formats. The kit provides pre-coated 384-well plates and optimized reagents for detecting low cGMP levels with a stable fluorescent readout and flexible timing for automation.
- Pre-coated, pre-blocked 384-well black well, clear bottom plates, two plates per kit
- Includes reagents and buffers for assay setup and detection, including antibody, conjugate, calibrator, and substrate
- Fluorescent Stoplight Red readout with stable signal from 10 minutes up to 24 hours
- Quantitative competitive immunoassay format suitable for cell-based assays and HTS
- Robust format resists interference from colored or fluorescent test compounds
Molecular Devices Reporter Assay Kit, Dual Luciferase, 1,000 Reactions
SpectraMax DuoLuc Reporter Assay Kit enables sensitive, quantitative measurement of firefly and Renilla luciferase activities in mammalian cells using flash-type luminescence. The bulk format supports high-throughput workflows and is optimized for use with microplate readers equipped with injectors. Preconfigured reader protocols simplify data acquisition and normalization to internal controls.
- Dual luciferase assay for firefly and Renilla measurements.
- Bulk kit provides reagents for 1,000 reactions.
- Flash-type luminescence chemistry for high sensitivity.
- Optimized for microplate readers with injector capability.
- Enables normalization to an internal control reporter.
- Includes assay reagents; additional passive lysis buffer available separately.
Cell Biolabs Inc CytoSelect™ 96-Well Phagocytosis Assay Kit (E. coli, Colorimetric), 96 assays
Phagocytosis comprises a series of events, starting with the binding and recognition of particles by cell surface receptors, followed by the formation of actin-rich membrane extensions around the particle. Cell Biolabs’ CytoSelect™ 96-well Phagocytosis Assay (E. coli) uses enzyme-labeled, E. coli particles as a phagocytosis pathogen; however, it does not involve subjective manual counting of E. coli particles inside cells. Instead external particles are blocked before the colorimetric detection of engulfed E. coli particles. This format provides a quantitative, high-throughput method to accurately measure phagocytosis. The CytoSelect™ 96-well Phagocytosis Assay (E. coli) provides a robust system for screening TLR ligands, phagocytosis activators or inhibitors. Each kit provides sufficient quantities to perform 96, 48, 24 tests in a 96, 48, or 24-well plate, respectively.
